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生理的酸素条件に曝露されたヒト細胞におけるキャップ結合タンパク質の解析
Kapitel
- 00:05Titel
- 01:03Subculturing Cells and Physioxic Exposure
- 01:57Cell Lysis
- 05:06Cap-binding Assay
- 08:44Results: Analysis of Cap-binding Ability in Response to Oxygen of eIF4E and eIF4E2 in an m7GTP Affinity Column
- 09:50Conclusion
Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.
Tags
Cap-binding ProteinsPhysiological Oxygen ConditionsAgarose Linked Seven Methyl-guanosine Cap AnalogCap-binding FactorsProtein SynthesisOxygen Regulated FactorsCap Dependent TranslationHypoxiaCancer TherapyTumor ProgressionNormaxiaCell CultureLysis BufferProtease Inhibitor Cocktail4-benzenesulfonyl Fluoride HydroclorideTrypsin EDTA
