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Culture de murines embryonnaires Métatarsiens: un modèle physiologique de endochondrale ossification
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
DOI:

07:23 min

December 03, 2016

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Kapitel

  • 00:05Titel
  • 00:51Dissection Preparation
  • 01:47Murine Embryonic Metatarsal Dissection and Culture
  • 04:57Results: A Tool for Understanding Endochondral Ossification
  • 06:20Conclusion

Summary

Automatische Übersetzung

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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