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一个简单的荧光试验犬中性粒细胞胞外陷阱里放的量化
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
Please note that all translations are automatically generated. Click here for the English version.

一个简单的荧光试验犬中性粒细胞胞外陷阱里放的量化

7,921 Views

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06:45 min

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November 21, 2016

DOI:

10.3791/54726-v

DOI:
10.3791/54726-v

•

06:45 min
November 21, 2016

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2 Views
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1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine,Iowa State University, 2MRC Centre for Inflammation Research,University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University

Summary

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中性粒细胞胞外陷阱（英语教师）是DNA，组蛋白和中性粒细胞的蛋白质网络。虽然先天免疫反应的一个组成部分，母语有牵连的自身免疫和血栓形成。这个协议描述了犬嗜中性粒细胞的分离和使用微板荧光测定教师的量化的简单方法。

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Jeffery, U., Gray, R. D., LeVine, D. N. A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release. J. Vis. Exp. (117), e54726, doi:10.3791/54726 (2016).

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