Journal
/
Immunologie und Infektion
/
Snelle en specifieke beoordeling van de halogenerend Peroxidase activiteit in leukocyten verrijkte bloedmonsters
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Please note that all translations are automatically generated. Click here for the English version.

Snelle en specifieke beoordeling van de halogenerend Peroxidase activiteit in leukocyten verrijkte bloedmonsters

10,164 Views

•

05:17 min

•

July 28, 2016

DOI:

10.3791/54484-v

DOI:
10.3791/54484-v

•

05:17 min
July 28, 2016

•

6 Views
, , , , ,
1Institute for Medical Physics and Biophysics, Medical Faculty,University of Leipzig, 2Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

Summary

Automatically generated

This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

Explore More Videos

Blood SampleLeukocyte EnrichmentHypotonic LysisPeroxidase ActivityAPF StainingHeme Peroxidase InhibitorHydrogen PeroxideCentrifugationHBSSCell Analysis

Read Article

Cite this Article

Flemmig, J., Schwarz, P., Bäcker, I., Leichsenring, A., Lange, F., Arnhold, J. Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples. J. Vis. Exp. (113), e54484, doi:10.3791/54484 (2016).

Download .ris file

Copy

Share Video

.

Copy