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Kapitel
- 00:05Titel
- 01:03Membrane Sheet Preparation and Fixation
- 02:53Photoactivation Localization Microscopy (PALM) Image Acquisition
- 05:40Results: Images of PI(4,5)P2 and iRFP-PAmCherry1-PHPLCδ1 Labeled INS-1 Cells
- 06:56Conclusion
PI (4,5) P2 regulerer forskellige cellulære funktioner, men dens nanoskala organisation i cellen plasmamembranen er dårligt forstået. Ved mærkning PI (4,5) P2 med en tofarvet fluorescerende probe fusioneret til pleckstrin homologidomænet beskriver vi en ny tilgang til at studere PI (4,5) P2 rumlige fordeling i plasmamembranen på nanometerskala .
Tags
Single-molecule Super-resolution ImagingPhosphatidylinositol 45-bisphosphatePlasma MembraneFluorescent ProbesNanoscale DistributionPhosphoinositide SignalingHigh Spatial ResolutionDetergent-freePlasma Membrane Sheet PreparationPhosphoinositide LabelingSingle-molecule Fluorescence ImagingSingle-molecule LocalizationTIRF MicroscopySMLM
