This content is Free Access.
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
9,193 Views
•
07:26 min
•
October 15, 2016
DOI:
Summary
Automatically generated
PI(4,5)P2 regulates various cellular functions, but its nanoscale organization in the cell plasma membrane is poorly understood. By labeling PI(4,5)P2 with a dual-color fluorescent probe fused to the Pleckstrin Homology domain, we describe a novel approach to study the PI(4,5)P2 spatial distribution in the plasma membrane at the nanometer scale.
Explore More Videos
Single-molecule Super-resolution ImagingPhosphatidylinositol 45-bisphosphatePlasma MembraneFluorescent ProbesNanoscale DistributionPhosphoinositide SignalingHigh Spatial ResolutionDetergent-freePlasma Membrane Sheet PreparationPhosphoinositide LabelingSingle-molecule Fluorescence ImagingSingle-molecule LocalizationTIRF MicroscopySMLM
Read Article
Cite this Article
Ji, C., Lou, X. Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes. J. Vis. Exp. (116), e54466, doi:10.3791/54466 (2016).
Copy