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在质膜用新型荧光探针磷脂酰-4,5-二磷酸的单分子超分辨率成像
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October 15, 2016
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PI（4,5）P2调节各种细胞功能，但其在细胞质膜的纳米级组织知之甚少。由标记的PI（4,5）P 2与融合于普列克底物蛋白同源结构域的双色荧光探针，我们描述了一种新的方法来研究的PI（4,5）P2在质膜2的空间分布在纳米尺度。
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Single-molecule Super-resolution ImagingPhosphatidylinositol 45-bisphosphatePlasma MembraneFluorescent ProbesNanoscale DistributionPhosphoinositide SignalingHigh Spatial ResolutionDetergent-freePlasma Membrane Sheet PreparationPhosphoinositide LabelingSingle-molecule Fluorescence ImagingSingle-molecule LocalizationTIRF MicroscopySMLM
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Ji, C., Lou, X. Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes. J. Vis. Exp. (116), e54466, doi:10.3791/54466 (2016).
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