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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

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11:25 min

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February 22, 2016

DOI:

10.3791/53654-v

DOI:
10.3791/53654-v

•

11:25 min
February 22, 2016

•

2 Views
, , , ,
1Developmental Biology Program,Sloan Kettering Institute

Summary

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该协议提出对蛋白质表达的原位分析进行定量，单细胞研究在小鼠植入前胚胎沿袭规范的方法。必要的囊胚的汇编程序，整个安装蛋白的免疫检测，在一个共焦显微镜，和核分割和图像分析的样品的成像进行描述。

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Saiz, N., Kang, M., Schrode, N., Lou, X., Hadjantonakis, A. Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos. J. Vis. Exp. (108), e53654, doi:10.3791/53654 (2016).

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