Journal
/
Entwicklungsbiologie
/
集成的无人类诱导多能干细胞的生成方法头发衍生角质形成细胞
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Please note that all translations are automatically generated. Click here for the English version.

集成的无人类诱导多能干细胞的生成方法头发衍生角质形成细胞

11,229 Views

•

08:36 min

•

August 20, 2015

DOI:

10.3791/53174-v

DOI:
10.3791/53174-v

•

08:36 min
August 20, 2015

•

12 Views
, ,
1Centre for Eye Research Australia & Department of Ophthalmology,University of Melbourne

Summary

Automatically generated

This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

Explore More Videos

Hair-derived KeratinocytesHuman Induced Pluripotent Stem CellsHiPSCsNon-invasiveReprogrammingEpisomal VectorsRegenerative MedicineDisease ModelingIn Vitro Drug ScreeningGene Therapy

Read Article

Cite this Article

Hung, S. S., Pébay, A., Wong, R. C. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J. Vis. Exp. (102), e53174, doi:10.3791/53174 (2015).

Download .ris file

Copy

Share Video

.

Copy