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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

DOI:
10.3791/53611-v

•

12:14 min

•

March 09, 2016

•

, ,
1Department of Microbiology,Southern California Costal Water Research Project Authority

Kapitel

  • 00:05Titel
  • 00:56Assay Mixture Preparation
  • 02:24Droplet Generation and PCR Plate Setup
  • 05:17Thermal Cycling and Droplet Reading
  • 08:01Data Analysis and Reporting
  • 09:46Results: Duplex Digital PCR is a Robust Assay for Simultaneous Quantification of General and Human-associated Fecal Contamination
  • 11:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

Tags

Duplex Digital PCR AssayEnterococcus Spp.HF183 MarkerFecal ContaminationWater Quality MonitoringQPCRMaster MixDroplet GenerationPositive ControlsNo Template Controls (NTCs)

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