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Immunologie und Infektion
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Utvikling av en<em> In vitro</em> Modellsystem for å studere samspillet mellom<em> Equus</em><em> Caballus</em> IgE med sin high-affinitetsreseptor FceRI
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JoVE Journal Immunologie und Infektion
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Utvikling av en<em> In vitro</em> Modellsystem for å studere samspillet mellom<em> Equus</em><em> Caballus</em> IgE med sin high-affinitetsreseptor FceRI

DOI:
10.3791/52222-v

•

07:31 min

•

November 01, 2014

•

, ,
1Biological Department,King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology,The University of Sheffield

Kapitel

  • 00:00Titel
  • 00:10Introduction
  • 01:14Animation
  • 02:37Washing
  • 03:22Antigen Challenge
  • 03:48Substrate
  • 04:23Stopping the Reaction
  • 04:47Reading
  • 04:54Calculations
  • 06:22Ergebnisse
  • 07:02Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Tags

Equine IgEFcεRIIn Vitro ModelRBL-2H3.1 CellsTransfectionAllergic Responseβ-hexosaminidase ReleaseDiagnostic ToolTherapeutic Intervention

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