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Methoden voor de kwantitatieve detectie van antilichaam-geïnduceerde complement activatie op de rode bloedcellen
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Methoden voor de kwantitatieve detectie van antilichaam-geïnduceerde complement activatie op de rode bloedcellen

30,195 Views

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06:29 min

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January 29, 2014

DOI:

10.3791/51161-v

DOI:
10.3791/51161-v

•

06:29 min
January 29, 2014

•

7 Views
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1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Tags

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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Meulenbroek, E. M., Wouters, D., Zeerleder, S. Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells. J. Vis. Exp. (83), e51161, doi:10.3791/51161 (2014).

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