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Journal
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Biologie
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卡哈尔(ICC)的网络的间质细胞在小鼠体内的可视化
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
Please note that all translations are automatically generated.
Click here for the English version.
卡哈尔(ICC)的网络的间质细胞在小鼠体内的可视化
DOI:
10.3791/2802-v
•
09:45 min
•
July 27, 2011
•
Yu Chen
2
,
Tambudzai Shamu
,
Hui Chen
,
Peter Besmer
,
Charles L. Sawyers
4
,
Ping Chi
5
1
Department of Medicine
,
Memorial Sloan Kettering Cancer Center
,
2
Human Oncology and Pathogenesis Program
,
Memorial Sloan Kettering Cancer Center
,
3
Developmental Biology Program
,
Memorial Sloan Kettering Cancer Center
,
4
Howard Hughes
,
Medical Institute
,
5
Laboratory of Chromatin Biology and Epigenetics
,
The Rockefeller University
Kapitel
00:05
Titel
01:35
Dissection of the Mouse Gastrointestinal Tract
02:56
Whole Mount Sample Preparation and Immunostaining
05:10
Cryosection Preparation and Immunostaining
07:44
Representative Imaging Results
09:23
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
Cajal间的间质细胞(ICC)的胃肠道(GI)的起搏细胞。他们之间的平滑肌细胞和调节胃肠道收缩后神经节神经元纤维形成的复杂的网络。在这里,我们目前的横断面和整个安装小鼠ICC网络的可视化的免疫方法。
Tags
Visualization
Interstitial Cells Of Cajal (ICC)
Mice
Gastrointestinal (GI) Tract
Pacemaker Cells
Slow Waves
Peristalsis
Enteric Nervous System
Motility Disorders
KIT Receptor Tyrosine Kinase
KIT Immunostaining
ICC Network
KIT Activity
Neoplastic Transformation
Gastrointestinal Stromal Tumor (GIST)
ETV1 Survival Factor
ICC/GIST Lineage
Tumorigenesis
Activating KIT Mutations
Visualization Methods
Previously Published Protocols
Anoctamin 1 (ANO1)
Membrane Marker
Immunofluorescence
Article
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