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Journal
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Biologie
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快速PCR热循环利用微型热对流
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Rapid PCR Thermocycling using Microscale Thermal Convection
Please note that all translations are automatically generated.
Click here for the English version.
快速PCR热循环利用微型热对流
DOI:
10.3791/2366-v
•
09:02 min
•
March 05, 2011
•
Radha Muddu
,
Yassin A. Hassan
,
Victor M. Ugaz
1
Department of Mechanical Engineering
,
Texas A&M University
,
2
Department of Mechanical Engineering and Department of Nuclear Engineering
,
Texas A&M University
,
3
Department of Chemical Engineering
,
Texas A&M University
Kapitel
00:05
Titel
01:21
Basic Design of the Convective Thermocycler
02:48
Preparation and Loading of the PCR Reaction Mixture
03:42
Running the Reaction in the Convective Thermocycler
04:25
Performing Gel Electrophoresis Analysis
05:31
Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
08:33
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
我们描述了一种新的方法,通过聚合酶链反应(PCR)进行DNA复制。热对流是利用变性,退火,以及延长条件之间不断穿梭试剂保持反对在反应器的表面温度恒定。这在本质上简单设计的承诺,使快速PCR更方便。
Tags
PCR Thermocycling
Rapid PCR
Microscale Thermal Convection
Polymerase Chain Reaction
Thermal Convection
PCR Thermocycling Hardware
Thermoelectric Heaters
Reaction Speed
Electrical Power
Portable Format
Convective Flows
Buoyancy Driven Instability
Spatial Temperature Gradient
Reactor Geometry
Denaturing Stage
Annealing Stage
Extension Stage
Article
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