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Rapid PCR Thermocycling using Microscale Thermal Convection
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Rapid PCR Thermocycling using Microscale Thermal Convection

Rapid PCR Thermocycling using Microscale Thermal Convection

22,396 Views

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09:02 min

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March 05, 2011

DOI:

10.3791/2366-v

DOI:
10.3791/2366-v

•

09:02 min
March 05, 2011

•

7 Views
, ,
1Makine Mühendisliği Bölümü,Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering,Texas A&M University, 3Department of Chemical Engineering,Texas A&M University

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We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.

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Cite this Article

Muddu, R., Hassan, Y. A., Ugaz, V. M. Rapid PCR Thermocycling using Microscale Thermal Convection. J. Vis. Exp. (49), e2366, doi:10.3791/2366 (2011).

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