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Crescimento e Diferenciação de Adultos Hippocampal terra ártico Squirrel células-tronco neurais
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Crescimento e Diferenciação de Adultos Hippocampal terra ártico Squirrel células-tronco neurais
Kapitel
- 00:05Titel
- 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
- 01:05Preparing poly-L-ornithine-coated Flasks
- 01:56Thawing and Seeding agsNSC
- 03:39Expanding agsNSC
- 04:11Trypsinization of Cells
- 05:27Inoculating Well Plates for Differentiation
- 07:25Maintenance of Neurons
- 08:09Results of Growth and Differentiation of agsNSC
- 09:29Conclusion
Células-tronco neurais foram preparadas a partir do hipocampo de adultos não-hibernando yearling esquilos do Ártico (AGS). Essas células-tronco neurais pode ser expandida através de numerosas passagens, diferenciados e mantidos como um neurônio quase 50:50 para a cultura da glia.
Tags
GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway
