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Journal / Developmental Biology / 胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养…
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JoVE Journal Developmental Biology
胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养
 
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胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养

Article DOI: 10.3791/65069-v • 07:44 min • March 3rd, 2023
March 3rd, 2023 •
1, 1, 1, 1
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences, University of Lisbon

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在这项工作中，优化了脱细胞方案以获得胎鼠骨骼肌的脱细胞基质。C2C12成肌细胞可以在这些基质中定植，增殖和分化。这种 体外 模型可用于研究骨骼肌疾病（如肌肉萎缩症）背景下的细胞行为。

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