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JoVE Journal Developmental Biology

从未受干扰和受伤的小鼠骨骼肌中分离和培养纤维脂肪祖细胞和肌肉干细胞的FACS-分离和培养

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从未受干扰和受伤的小鼠骨骼肌中分离和培养纤维脂肪祖细胞和肌肉干细胞的FACS-分离和培养

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022

June 8th, 2022 •

Giulia Riparini¹, James M. Simone², Vittorio Sartorelli¹

¹Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), ²Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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纤维脂肪祖细胞（FAPs）和肌肉干细胞（MuSCs）的精确鉴定对于研究它们在生理和病理条件下的生物学功能至关重要。该协议为从成年小鼠肌肉中分离，纯化和培养FAP和MuSC提供了指南。

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发育生物学，第184期，肌肉纤维成脂祖细胞，肌肉干细胞（MuSCs），间充质干细胞，间充质基质细胞，FACS，肌肉再生

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