Journal
/
发育生物学
/
从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
Please note that all translations are automatically generated. Click here for the English version.

从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征

15,618 Views

•

11:22 min

•

January 12, 2015

DOI:

10.3791/52049-v

DOI:
10.3791/52049-v

•

11:22 min
January 12, 2015

•

4 Views
, , , ,
1Centre of Human and Aerospace Physiological Sciences,King’s College London, 2Wellcome Trust-Medical Research Council,Cambridge Stem Cell Institute

Tags

Skeletal MuscleSatellite CellsMyogenic CellsMuscle Precursor CellsFibroblastsCD56DesminTE-7Enzymatic DigestionCollagenaseDispaseMagnetic Activated Cell Sorting (MACS)ImmunocytochemistryCell CultureMuscle Regeneration

Read Article

Cite this Article

Agley, C. C., Rowlerson, A. M., Velloso, C. P., Lazarus, N. L., Harridge, S. D. R. Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle. J. Vis. Exp. (95), e52049, doi:10.3791/52049 (2015).

Download .ris file

Copy

Share Video

.

Copy