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हीट-शॉक प्रोटीन 90 इनहिबिटर की खोज के लिए मैलाकाइट ग्रीन परख
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Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
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हीट-शॉक प्रोटीन 90 इनहिबिटर की खोज के लिए मैलाकाइट ग्रीन परख

3,656 Views

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07:57 min

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January 20, 2023

DOI:

10.3791/64693-v

DOI:
10.3791/64693-v

•

07:57 min
January 20, 2023

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5 Views
, , , , , ,
1Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), 2School of Life Sciences,University of Sussex

Tags

Malachite Green AssayHsp90ATPase ActivityPhosphate DetectionATP HydrolysisInhibitorsHigh-throughput ScreeningGeldanamycinSerial DilutionBlankPositive ControlAbsorbance

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Gupta, S. D., Song, D., Lee, S., Lee, J. W., Park, J., Prodromou, C., Pan, C. Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors. J. Vis. Exp. (191), e64693, doi:10.3791/64693 (2023).

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