Journal
/
生物化学
/
Malakitgrøn analyse til opdagelse af varmechokprotein 90-hæmmere
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Malakitgrøn analyse til opdagelse af varmechokprotein 90-hæmmere

3,656 Views

•

07:57 min

•

January 20, 2023

DOI:

10.3791/64693-v

DOI:
10.3791/64693-v

•

07:57 min
January 20, 2023

•

5 Views
, , , , , ,
1Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), 2School of Life Sciences,University of Sussex

Tags

Malachite Green AssayHsp90ATPase ActivityPhosphate DetectionATP HydrolysisInhibitorsHigh-throughput ScreeningGeldanamycinSerial DilutionBlankPositive ControlAbsorbance

Read Article

Cite this Article

Gupta, S. D., Song, D., Lee, S., Lee, J. W., Park, J., Prodromou, C., Pan, C. Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors. J. Vis. Exp. (191), e64693, doi:10.3791/64693 (2023).

Download .ris file

Copy

Share Video

.

Copy