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Journal / Cancer Research / Multiparametrisk tumor organoid narkotika…
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Cancer Research

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JoVE Journal Cancer Research
Multiparametrisk tumor organoid narkotika screening ved hjelp av Widefield Live-Cell Imaging for bulk og single-organoid analyse
 
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Multiparametrisk tumor organoid narkotika screening ved hjelp av Widefield Live-Cell Imaging for bulk og single-organoid analyse

Article DOI: 10.3791/64434-v • 12:41 min • December 23rd, 2022
December 23rd, 2022 •
1, 2, 1, 1, 1, 1,3, 2, 1,4, 1,4, 1,5, 1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

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Denne protokollen beskriver en semi-automatisert metode for middels til høy gjennomstrømning organoid narkotika screenings og mikroskop-agnostisk, automatisert bildeanalyse programvare for å kvantifisere og visualisere multiparametriske, single-organoid narkotika responser for å fange intratumor heterogenitet.

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Kreftforskning utgave 190 levende celleavbildning organoid narkotika screening multiparametrisk widefield bildebehandling etikettfri analyse tumoroider
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