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थोक और एकल-ऑर्गेनॉइड विश्लेषण के लिए वाइडफील्ड लाइव-सेल इमेजिंग का उपयोग करके मल्टीपैरामीट्रिक ट्यूमर ऑर्गेनॉइड ड्रग स्क्रीनिंग
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December 23, 2022
DOI:
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON),University of Antwerp, 2Industrial Vision Lab,University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery,University Hospital Antwerp (UZA), 4Department of Oncology,University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT),University of Antwerp
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MultiparametricTumor OrganoidDrug ScreeningLive-cell ImagingBulk AnalysisSingle-organoid AnalysisStreamlined WorkflowAutomated AnalysisClinically RelevantPatient-derived Tumor OrganoidsPDTOExtracellular MatrixECMDissociationCell SuspensionPDAC OrganoidCulture MediumCell PlatingECM DomesY-27632Organoid Harvesting
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Le Compte, M., Cardenas De La Hoz, E., Peeters, S., Smits, E., Lardon, F., Roeyen, G., Vanlanduit, S., Prenen, H., Peeters, M., Lin, A., Deben, C. Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis. J. Vis. Exp. (190), e64434, doi:10.3791/64434 (2022).
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