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Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

DOI:

09:24 min

October 12, 2022

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Chapters

  • 00:04Introduction
  • 00:52Candida albicans Strains and Animal Preparation
  • 02:08Hair Removal and Inoculation
  • 04:06Quantify In Vitro Morphogenesis for Comparison to In Vivo Results
  • 04:42Preparation for In Vivo Imaging
  • 06:37Imaging
  • 07:38Manual Two‐Dimensional Analysis: Frequency of Filamentation
  • 07:59Results: Analyzing In Vitro and In Vivo Morphology of the Mutant Strains
  • 08:51Conclusion

Summary

自动翻译

This manuscript describes a method for screening moderately sized Candida albicans mutant libraries for morphogenesis phenotypes during active infection in a mammalian host using non-invasive confocal microscopy.

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