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鸡颅神经嵴细胞培养物的制备及形态学分析
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
DOI:

09:26 min

June 27, 2022

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Chapters

  • 00:04Introduction
  • 01:06Embryo Incubation and Preparation of Culture Dishes
  • 01:49Isolation of Chick Embryos
  • 03:01Dissecting Neural Folds
  • 04:22Plating Neural Folds
  • 05:20Fixing and Staining of Cultured Migratory Neural Crest Cells for Morphological Analysis
  • 07:08Results: Assessment of Cultured Migratory Neural Crest Cells
  • 08:39Conclusion

Summary

自动翻译

这种多功能方案描述了通过从鸡胚胎中切除颅神经褶皱来分离前迁移神经嵴细胞(NCC)。在铺板和孵育时,迁移性NCC从神经折叠外植体中出现,允许在简化的2D环境中评估细胞形态和迁移。

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