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光诱导 位透射电子显微镜观察液-软物质相互作用
JoVE 杂志
工程学
Author Produced
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JoVE 杂志 工程学
Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
DOI:

05:33 min

July 26, 2022

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Chapters

  • 00:04Introduction
  • 00:41Encapsulation of Bacteria with the Photosensitizer
  • 02:07In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
  • 02:50In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
  • 03:51Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
  • 04:47Conclusion

Summary

自动翻译

本协议描述了使用光照明系统进行透射电子显微镜(TEM)修饰,制造液体细胞以及细菌细胞和光敏剂之间光诱导相互作用的 原位 TEM观察。还讨论了样品制备方法、电子束损伤和成像。

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