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骨髓来源巨噬细胞的分离和体外培养用于NO-氧化还原生物学研究

JoVE 杂志
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JoVE 杂志免疫与感染

Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

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骨髓来源巨噬细胞的分离和体外培养用于NO-氧化还原生物学研究

7,298 Views

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06:34 min

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May 31, 2022

DOI:

10.3791/62834-v

DOI:

10.3791/62834-v

•

06:34 min

May 31, 2022

•

58 Views

Marina Diotallevi*^1,2, Thomas Nicol*^1,2, Faseeha Ayaz^1,2, Jade Bailey^1,2, Andrew Shaw^1,2, Eileen McNeill^1,2, Ben Davies^1,2, Keith M. Channon^1,2, Mark J. Crabtree^1,2

¹BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital,University of Oxford, ²Wellcome Centre for Human Genetics,University of Oxford

Summary

该方案已被建立用于培养四氢生物蝶呤（BH4）-和诱导的一氧化氮合酶（iNOS）缺陷原代鼠巨噬细胞，以研究NO-氧化还原生物学。该研究的重点是减少传统分离和培养方法中发现的BH4和其他伪影的潜在污染，这些伪影可能会混淆实验结果和对结果的解释。

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