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Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis

Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis

DOI:
10.3791/62699-v

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08:27 min

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September 02, 2021

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,
1Geriatrics Research Education and Clinical Center,Veterans Affairs Puget Sound Health Care System, 2Division of Gerontology and Geriatric Medicine, Department of Medicine,University of Washington

Chapters

  • 00:04Introduction
  • 00:45Radial Locomotion Assay
  • 02:10Computer-Analyzed Swimming Assay
  • 05:51Data Analysis
  • 06:19Results: Analysis of the Crawling Speeds in Radial Locomotion and Thrashing in the Swimming Assay
  • 07:48Conclusion

Summary

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This protocol describes two sensitive assays for discriminating among mild, moderate, and severe motor impairment in C. elegans models of amyotrophic lateral sclerosis, with general utility for C. elegans strains, with altered motility.

Tags

C. ElegansAmyotrophic Lateral Sclerosis (ALS)Motor ImpairmentRadial Locomotion AssaySwimming AssayMotility PhenotypesMovement QuantificationComputer-based TrackingThrashing Movements

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