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Absorção de Vesículos Extracelulares Fluorescentes Em Vitro e na Medula Espinhal
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神经科学
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JoVE 杂志 神经科学
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Absorção de Vesículos Extracelulares Fluorescentes Em Vitro e na Medula Espinhal

3,281 Views

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09:01 min

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May 23, 2021

DOI:

10.3791/62537-v

DOI:
10.3791/62537-v

•

09:01 min
May 23, 2021

•

2 Views
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1Department of Pharmacology & Physiology,Drexel University College of Medicine

Summary

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Descrevemos um protocolo para rotular pequenas vesículas extracelulares derivadas do macrófago com corantes PKH e observar sua absorção in vitro e na medula espinhal após a entrega intratecal.

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Gupta, R., Luo, X., Lin, Z., Tian, Y., Ajit, S. K. Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord. J. Vis. Exp. (171), e62537, doi:10.3791/62537 (2021).

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