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Opname van fluorescerende gelabelde kleine extracellulaire blaasjes in vitro en in het ruggenmerg
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神经科学
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JoVE 杂志 神经科学
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
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Opname van fluorescerende gelabelde kleine extracellulaire blaasjes in vitro en in het ruggenmerg

3,281 Views

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09:01 min

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May 23, 2021

DOI:

10.3791/62537-v

DOI:
10.3791/62537-v

•

09:01 min
May 23, 2021

•

2 Views
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1Department of Pharmacology & Physiology,Drexel University College of Medicine

Summary

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We beschrijven een protocol om van macrofagen afgeleide kleine extracellulaire blaasjes te labelen met PKH-kleurstoffen en hun opname in vitro en in het ruggenmerg na intrathecale toediening te observeren.

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Gupta, R., Luo, X., Lin, Z., Tian, Y., Ajit, S. K. Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord. J. Vis. Exp. (171), e62537, doi:10.3791/62537 (2021).

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