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使用开源 smfBox 进行精确和准确的单分子 FRET 测量

JoVE 杂志
生物化学

This content is Free Access.

JoVE 杂志生物化学

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

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使用开源 smfBox 进行精确和准确的单分子 FRET 测量

3,047 Views

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07:12 min

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July 05, 2021

DOI:

10.3791/62378-v

DOI:

10.3791/62378-v

•

07:12 min

July 05, 2021

•

13 Views

Mahmoud A. S. Abdelhamid*¹, Alice V. Rhind-Tutt*¹, Benjamin Ambrose¹, Timothy D. Craggs¹

¹Sheffield Institute for Nucleic Acids, Department of Chemistry,University of Sheffield

Summary

本文提供了使用开源、廉价的 smfBox 对单个、自由扩散的生物分子进行完全校正的准确 FRET 测量的分步说明，从打开、通过对准和聚焦，再到数据收集和分析。

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