Journal
/
发育生物学
/
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

DOI:
10.3791/62326-v

•

07:32 min

•

February 17, 2021

•

, , ,
1Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School,University of Hull

Chapters

  • 00:05Introduction
  • 00:39Preparation of Skin for Wounding and Creating Ex Vivo Human Skin Wounds
  • 02:36Whole Mount Staining of Ex Vivo Wounds
  • 04:14Explant Imaging
  • 05:18Results: Wound Closure Evaluation by Whole-Mount Tissue Staining
  • 07:06Conclusion

Summary

自动翻译

自动翻译

Here we demonstrate an optimized technique for assessing wound repair using ex vivo human skin combined with a whole-mount staining approach. This methodology provides a pre-clinical platform for the evaluation of potential wound therapies.

Tags

Ex VivoHuman SkinWound ModelWhole-mount StainingSkin RepairWound AssessmentSkin ExplantWound HealingSkin FixativeFluorescent Staining

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Read Article