Here we demonstrate an optimized technique for assessing wound repair using ex vivo human skin combined with a whole-mount staining approach. This methodology provides a pre-clinical platform for the evaluation of potential wound therapies.
Wilkinson, H. N., Kidd, A. S., Roberts, E. R., Hardman, M. J. Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair. J. Vis. Exp. (168), e62326, doi:10.3791/62326 (2021).