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隔离和成像现场，通过颤音分割老鼠肾佩尔维斯的精致起搏器区域

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Please note that all translations are automatically generated. Click here for the English version.

隔离和成像现场，通过颤音分割老鼠肾佩尔维斯的精致起搏器区域

DOI:

10.3791/62040-v

•

07:38 min

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April 30, 2021

•

Nathan Grainger², Kenton M. Sanders, Bernard T. Drumm³

¹Department of Physiology & Cell Biology,University of Nevada, Reno School of Medicine, ²Department of Physiology & Membrane Biology,University of California School of Medicine, ³Department of Life & Health Sciences,Dundalk Institute of Technology

Chapters

00:05Introduction
00:44Preparation and Dissection of Kidney and Calibration of Vibratome Instrument
02:32Vibratome Sectioning and Kidney Slice Ca²⁺ Image Acquisition
04:06Kidney Slice Ca²⁺ Image Acquisition
05:14Results: Imaging of Spontaneous Calcium Ion Transient in Vibratome Sections of Whole Kidneys
07:01Conclusion

Summary

自动翻译

此协议的目标是使用振动切除隔离小鼠肾骨盆的完整心脏起搏器区域。然后，这些部分可用于原位 Ca²⁺ 成像，以阐明心脏起搏器细胞和振动切片中其他间质细胞的 Ca²⁺ 瞬态特性。

Tags

Pacemaker Regions Renal Pelvis Vibratome Sectioning Intact Preparation Smooth Muscle Kidney Ureter Calcium Ion Imaging Dissection Razor Blade KRB Solution

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