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Transpupillær to-foton In vivo Billeddannelse af musens nethinde
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Transpupillær to-foton In vivo Billeddannelse af musens nethinde

DOI:
10.3791/61970-v

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09:03 min

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February 13, 2021

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, ,
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, 2Graduate Program in Neuroscience,Washington University School of Medicine, 3Department of Neuroscience,Washington University School of Medicine, 4Hope Center for Neurological Disorders,Washington University School of Medicine

Chapters

  • 00:05Introduction
  • 00:42Imaging Preparation
  • 03:44Two-Photon Image Acquisition
  • 06:13Mouse Recovery
  • 06:46Results: Representative In Vivo Mouse Retina Imaging
  • 08:36Conclusion

Summary

自动翻译

自动翻译

In vivo billeddannelse er et kraftfuldt værktøj til undersøgelse af biologi i sundhed og sygdom. Denne protokol beskriver transpupillær billeddannelse af musens nethinde med et standard to-fotonmikroskop. Det demonstrerer også forskellige in vivoimaging metoder til fluorescerende mærkning af flere cellulære kohorter i nethinden.

Tags

Keywords: In Vivo ImagingTwo-photon MicroscopyMouse RetinaOphthalmological DiseasesRetinal CellsStabilizationHead HolderPupil DilationEar Canal PinsBite BarEye LubricantFluorescenceRetinal Structures

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