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Rastgele Mikrotohum Matris Taraması Ile Protein Kristallerinin I3C ile Türevleştirilmesi
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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Rastgele Mikrotohum Matris Taraması Ile Protein Kristallerinin I3C ile Türevleştirilmesi

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14:04 min

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January 16, 2021

DOI:

10.3791/61894-v

DOI:
10.3791/61894-v

•

14:04 min
January 16, 2021

•

6 Views
, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Summary

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Bu makalede, seyrek matris ekranlarda yeni kristalleşme koşulları oluşturmak için mikrotohumlama kullanarak I3C (5-amino-2,4,6-triiodoishthalic asit) ile türemiş protein kristalleri oluşturmak için bir yöntem sunar. Tepsiler sıvı dağıtım robotları kullanılarak veya elle kurulabilir.

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Cite this Article

Truong, J. Q., Nguyen, S., Bruning, J. B., Shearwin, K. E. Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening. J. Vis. Exp. (167), e61894, doi:10.3791/61894 (2021).

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