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Derivazione di cristalli proteici con I3C utilizzando lo screening casuale della matrice microseed
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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Derivazione di cristalli proteici con I3C utilizzando lo screening casuale della matrice microseed

4,372 Views

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14:04 min

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January 16, 2021

DOI:

10.3791/61894-v

DOI:
10.3791/61894-v

•

14:04 min
January 16, 2021

•

6 Views
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1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Summary

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Questo articolo presenta un metodo per generare cristalli proteici derivati con I3C (acido 5-ammino-2,4,6-triiodoisoftalico) utilizzando il microseeding per generare nuove condizioni di cristallizzazione negli schermi a matrice sparsa. I vassoi possono essere impostati utilizzando robot di erogazione di liquidi o a mano.

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Cite this Article

Truong, J. Q., Nguyen, S., Bruning, J. B., Shearwin, K. E. Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening. J. Vis. Exp. (167), e61894, doi:10.3791/61894 (2021).

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