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Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

DOI:
10.3791/61683-v

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07:43 min

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August 05, 2021

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, , , , ,
1College of Veterinary Medicine, Department of Small Animal Clinical Sciences,Michigan State University, 2Lineage Cell Therapeutics, Inc.

Chapters

  • 00:04Introduction
  • 00:51Subretinal Space Preparation
  • 04:50Subretinal Organoid Implantation
  • 06:06Results: Representative Subretinal Organoid Implantation
  • 07:04Conclusion

Summary

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Presented here is a surgical technique for transplanting human pluripotent stem cell (hPSC)-derived retinal tissue into the subretinal space of a large animal model.

Tags

Subretinal TransplantationFeline Large Animal ModelRetinal DegenerationAge-related Macular DegenerationRetinitis PigmentosaLeber Congenital AmaurosisLateral CanthotomyBarraquer Eyelid SpeculumCastroviejo Corneal Tying ForcepsMosquito HemostatSilk SuturesTenon’s CapsuleSclerotomyPolyglactin Sutures

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