This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.
Wacker, I. U., Veith, L., Spomer, W., Hofmann, A., Thaler, M., Hillmer, S., Gengenbach, U., Schröder, R. R. Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes. J. Vis. Exp. (133), e57059, doi:10.3791/57059 (2018).