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Biochemistry

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JoVE Journal Biochemistry
In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans
 
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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Article DOI: 10.3791/60910-v • 08:00 min • April 1st, 2020
April 1st, 2020 •
1, 1
1Department of Pharmaceutical Sciences, University of Maryland

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In vivo l’oxydation photochimique rapide des protéines (IV-FPOP) est une technique d’empreinte de protéine radicale hydroxyl qui permet la cartographie de la structure des protéines dans leur environnement indigène. Ce protocole décrit l’assemblage et la mise en place du système de flux microfluidique IV-FPOP.

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Biochimie Numéro 158 Caenorhabditis elegans empreinte de protéines radicales hydroxyl FPOP spectrométrie de masse in vivo protéomique
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