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Biochemistry

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JoVE Journal Biochemistry
In Vivo Hydroxyl Radical Protein Footprinting for studiet av proteininteraksjoner i Caenorhabditis elegans
 
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In Vivo Hydroxyl Radical Protein Footprinting for studiet av proteininteraksjoner i Caenorhabditis elegans

Article DOI: 10.3791/60910-v • 08:00 min • April 1st, 2020
April 1st, 2020 •
1, 1
1Department of Pharmaceutical Sciences, University of Maryland

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In vivo rask fotokjemisk oksidasjon av proteiner (IV-FPOP) er en hydroksylradikal proteinfotavtrykksteknikk som gjør det mulig å kartlegge proteinstruktur i sitt eget miljø. Denne protokollen beskriver montering og oppsett av IV-FPOP mikrofluidic flow system.

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Biokjemi Utgave 158 Caenorhabditis elegans hydroksylradikal proteinfotavtrykk FPOP massespektrometri in vivo proteomics
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