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Pinces optiques pour étudier les interactions ARN-protéine dans la régulation de la traduction

JoVE 杂志
生物化学
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JoVE 杂志生物化学

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

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Pinces optiques pour étudier les interactions ARN-protéine dans la régulation de la traduction

4,418 Views

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12:26 min

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February 12, 2022

DOI:

10.3791/62589-v

DOI:

10.3791/62589-v

•

12:26 min

February 12, 2022

•

11 Views

Lukas Pekarek, Stefan Buck, Neva Caliskan²

¹Helmholtz Institute for RNA-based Infection Research (HIRI),Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), ²Medical Faculty,Julius-Maximilians University Würzburg

Tags

Optical Tweezers RNA-protein Interactions Translation Regulation Single Molecule Techniques Fluorescence Assisted Optical Tweezers Force-ramp Experiments Constant Force Measurements Real-time Visualization DNA-RNA Construct In-vitro Transcription PCR Reactions

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