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Eliminación de genes sin marcadores por Floxed Cassette Allelic Exchange Mutagenesis en Chlamydia trachomatis
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Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Eliminación de genes sin marcadores por Floxed Cassette Allelic Exchange Mutagenesis en Chlamydia trachomatis

6,450 Views

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10:35 min

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January 30, 2020

DOI:

10.3791/60848-v

DOI:
10.3791/60848-v

•

10:35 min
January 30, 2020

•

8 Views
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1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Summary

Automatically generated

Aquí se describe un método para la eliminación de genes dirigida y sin marcadores en Chlamydia trachomatis mediante mutagénesis de intercambio alélico de casete floxed, FLAEM.

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Cite this Article

Keb, G., Fields, K. A. Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis. J. Vis. Exp. (155), e60848, doi:10.3791/60848 (2020).

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