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Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
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发育生物学
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JoVE 杂志 发育生物学
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

13,868 Views

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10:24 min

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May 24, 2020

DOI:

10.3791/60843-v

DOI:
10.3791/60843-v

•

10:24 min
May 24, 2020

•

4 Views
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1Center for Molecular Medicine,University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

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Human Pluripotent Stem CellsSympathetic NeuronsFeeder-freeChemically DefinedEfficient DifferentiationElectrical ActivityDisease ModelingRegenerative MedicineDrug ScreeningCo-culture System

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Wu, H. F., Zeltner, N. Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions. J. Vis. Exp. (159), e60843, doi:10.3791/60843 (2020).

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