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使用免疫荧光显微镜鉴定帕芬-嵌入式猫动脉血栓中中性粒细胞外陷阱
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JoVE 杂志 免疫与感染
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
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使用免疫荧光显微镜鉴定帕芬-嵌入式猫动脉血栓中中性粒细胞外陷阱

7,790 Views

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08:48 min

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March 29, 2020

DOI:

10.3791/60834-v

DOI:
10.3791/60834-v

•

08:48 min
March 29, 2020

•

10 Views
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1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis

Summary

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我们描述了一种使用热诱导抗原检索和双免疫标记协议识别甲醛固定和石蜡嵌入的猫科动物心性动脉血栓中嗜中性粒细胞外陷阱（NETs）的方法。

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Cite this Article

Duler, L., Nguyen, N., Ontiveros, E., Li, R. H. L. Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy. J. Vis. Exp. (157), e60834, doi:10.3791/60834 (2020).

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