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癌症研究
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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

DOI:
10.3791/60316-v

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09:29 min

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March 20, 2020

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, , , , , , ,
1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research,National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research,Leidos Biomedical Research, Inc.

Chapters

  • 00:05Introduction
  • 00:55Orthotopic Breast Cancer Model and Tumor Size Measurement
  • 02:05In Vivo Bioluminescence and Ex Vivo Fluorescence Imaging
  • 04:19Molecular Metastatic Breast Cancer Cell Detection
  • 06:27Lung Metastatic Breast Cancer Cell Detection
  • 07:29Results: Representative Bioluminescence Imaging (BLI) and GFP Signal Detection
  • 08:52Conclusion

Summary

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This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

Tags

Triple Negative Breast CancerOrthotopic Breast Cancer ModelGFP TagBioluminescence ImagingMicrometastasisPCR AnalysisAnti-cancer AgentsEMTDrug ResistanceCancer Stem Cell MechanismsMetastatic ProcessAnti-cancer Drug DevelopmentXenograftTumor Size MeasurementLuciferin KineticsLung MetastasisBrain Metastasis

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