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Generatie chimeric Axolotls met Mutant Haploid Ledematen door embryonale grafting
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
DOI:

07:17 min

January 29, 2020

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Chapters

  • 00:05Title
  • 00:48Female Gamete Donor Preparation
  • 01:36Male Gamete Collection and Preparation
  • 02:51Female Gamete Collection
  • 03:48Haploid-Diploid Chimera Generation
  • 05:07Results: Representative Haploid and Diploid Limb Development
  • 06:30Conclusion

Summary

自动翻译

Dit doel van dit protocol is het produceren van chimerische axolotls met haploïde voorpoten afgeleid van Cas9-mutagenized donorweefsel met behulp van embryonale weefsel enten technieken.

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