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JoVE Journal Biochemistry

通过红外纳米光谱和原子力显微镜对单个蛋白质聚合进行特征化

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通过红外纳米光谱和原子力显微镜对单个蛋白质聚合进行特征化

Article DOI: 10.3791/60108-v • 12:58 min • September 12th, 2019

September 12th, 2019 •

Francesco Simone Ruggeri¹, Tomas Šneideris^1,2, Sean Chia¹, Michele Vendruscolo¹, Tuomas P. J. Knowles^1,3

¹Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, ²Institute of Biotechnology, Life Sciences Center, Vilnius University, ³Cavendish Laboratory, Department of Physics, University of Cambridge

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我们描述了红外纳米光谱学和高分辨率原子力显微镜的应用，将蛋白质自组装过程可视化为寡聚体和淀粉样纤维蛋白，这与发病和发展密切相关广泛的人类神经退行性疾病。

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生物化学，问题 151，淀粉样蛋白，神经变性，蛋白质聚合，原子力显微镜，红外纳米光谱，单分子分析，光谱

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