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Målretning Alpha Synuclein aggregater i kutane perifer nerve fibre af Free-flydende immunofluorescens analyse
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
DOI:

08:33 min

June 25, 2019

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Chapters

  • 00:04Title
  • 01:17Tissue Fixation and Storage
  • 02:15Tissue Cut with a Cryotome
  • 03:11Immunofluorescence Staining
  • 05:34Immunofluorescence Imaging
  • 06:01Results: Analysis of the Free-floating Immunofluorescence Assay
  • 08:09Conclusion

Summary

自动翻译

Her præsenterer vi en protokol for en fritflydende indirekte immunofluorescens assay på hud biopsi sektioner, der giver mulighed for identifikation af sygdomsspecifikke kropsbygnings varianter af alpha synuclein involveret i Parkinsons sygdom og flere proteiner i perifert nervesystem.

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