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JoVE Journal Biochemistry
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
 

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Article DOI: 10.3791/59215-v • 10:05 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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We present experimental approaches for studying RNA-interactors of double-stranded RNA binding protein kinase RNA-activated (PKR) during the mammalian cell cycle using HeLa cells. This method utilizes formaldehyde to crosslink RNA-PKR complexes and immunoprecipitation to enrich PKR-bound RNAs. These RNAs can be further analyzed through high-throughput sequencing or qRT-PCR.

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Keywords: RNA Interactors Protein Kinase RNA-Activated Mammalian Cell Cycle Crosslinking Formaldehyde Immunoprecipitation Neurodegenerative Disease Parkinson's Disease Alzheimer's Disease Double-stranded RNA RNA-binding Proteins Post-transcriptional Regulation Gene Expression Cell Cycle Arrest HeLa Cells Thymidine Nocodazole
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